The Effect Of Ph On Enzyme Activity

The Effect of pH on Function of Catecholase Enzyme in white potato vine Abstract In biochemical reactions, enzymes activity could be qualifyingd ground on these factors: parsimoniousness of subst considers, salt concentration, al loseeric regulators, temperature and pH. Inventing the hypothesis is change magnitude concentration of pH would annex the activity of enzyme Catecholase and the rate of reaction. Therefore, the purpose of this experiment was to test the frame of pH on Catecholase (one kind of enzymes in potato) activity by using pH12 buffer. By using the Spectronic 20D+ Spectrophotometer, the change in absorbance everyplace time push aside be recorded. The result proved: trend to the hypothesis, at pH 12, activity of enzyme Catecholase and the rate of reaction decreased. Introduction pH is a measure of the concentration of heat content H+ in origin so the higher concentration hydrogen ion, the frown pH. pH is one of the most important factors affect to human body. pH was divided three parts on pH home base from pH 0 to pH 14: at pH 7, solutions are neutral. pH > 7 is alkalinity purlieu that pass on restrict hydrogen ion, and pH < 7 is acidic environment that willing donate hydrogen ion. On the other hand, increasing hydrogen ion concentration will reduce the bound surface cation concentration.

diminish hydrogen ion concentration will increase the hydroxyl ascendent ion concentration (M. Chaplin, 2004). Enzymes are one of the most efficient catalysts in biochemical reactions. They are used to speed up the reaction rates by providin g an alternative reaction pathway of turn ! away activation energy, which is the minimum energy that requires for reactions occur. Catalysts take part in a reaction (chemical involved), but they do not provide any changes in chemical reaction. (Farabee, 2001) Enzymes are proteins made of a combination of different amino acids. Therefore, the enzymes may be befuddled their function when structure of protein is changed under conditions such as cofactors, coenzymes,...If you indirect request to get a full essay, order it on our website: OrderCustomPaper.com

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